High Purity 99% Tris Base Buffer CAS 1185-53-1

Tianjin Gnee Biotech Co., Ltd. is one of the leading manufacturers and suppliers of high purity 99% tris base buffer cas 1185-53-1 in China. Welcome to buy cheap chemical products for sale here from our factory. For price consultation, contact us.

 

Application of Tris Base Buffer：
Used for biological buffer solutions. The pH value of all buffers depends on temperature and concentration. For Tris buffer solutions, the pH increases by approximately 0.03 units for every 1°C increase in temperature, and decreases by about 0.03–0.05 units for every tenfold dilution.

 

 

CAS NO.：	1185-53-1
Appearance:	white crystal
Content:	≥99%
Melting point:	150±2℃
Moisture:	≤1.0%
Heavy metal (pb):	5ppm
Uses:	intermediate for organic synthesis.
Packing:	fiber drum lined with plastic bag, net weight 25kg/drum

 

Function of Tris Base Buffer：

1. Maintaining a Stable pH Environment
One of the most prominent functions of Tris-HCl is its ability to effectively maintain the pH stability of lysis buffers. Tris-HCl has a broad buffering range (typically between pH 7.0–9.0), allowing it to effectively resist interference from external acidic or alkaline substances. This provides a stable pH environment for biomolecules, ensuring they retain their native structure and functional properties during the lysis process. This product is formulated using high-purity tris base buffer and tris base HCl, offering enhanced buffering performance suitable for various molecular biology applications.

2. Promoting Cell Lysis
Although Tris-HCl does not directly disrupt cell membranes, it indirectly promotes efficient cell lysis through synergistic interactions with other lysis components, such as detergents and protease inhibitors. The buffer system is based on a tris base tris HCl combination, which improves lysis efficiency and compatibility with different sample types.

3. Protecting Biomolecules from Degradation
Through its buffering capacity, Tris-HCl not only inhibits the activity of degrading enzymes but also minimizes conformational changes in biomolecules caused by pH fluctuations. This helps protect DNA, RNA, and proteins from degradation. Especially effective in pH-sensitive sample handling, the combined use of tris base and tris buffer further enhances system stability.

4. Optimizing Subsequent Experimental Steps
The use of Tris-HCl in lysis buffers also facilitates downstream molecular procedures such as DNA purification, PCR amplification, and electrophoresis. Its stable pH and ionic strength help reduce nonspecific binding and precipitation in subsequent steps, improving experimental efficiency and reproducibility. Additionally, the buffering capacity of Tris-HCl helps counteract pH fluctuations introduced during later processing steps, ensuring biomolecular stability throughout the experimental workflow.

 

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