Tris Base /Tris (hydroxymethyl) Aminomethane THAM CAS 77-86-1
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Tris Hydroxymethyl Aminomethane (THAM) is an organic substance, a chemical formula C4H11NO3, with white crystalline particles.
Tris Hydroxymethyl Aminomethane (THAM) can be used for the preparation of buffer solution in biochemistry and molecular biology experiment, pharmaceutical intermediate, surfactant and curing accelerator, water-soluble polymer containing Tris structure unit as paint dispersant, formaldehyde adsorption material in the room, etc.
| Item | Pharma grade |
| Appearance | White crystal powder |
| Solubility | Clear, colorless solution |
| Assay | ≥99.50% |
| Moisture | ≤0.30% |
| Insolubles | ≤0.005% |
| PH(5%) | 10.0-11.5 |
| UV Absorbance(290nm,40% aq.) | ≤0.1 |
| UV Absorbance(400nm 40% aq.) | ≤0.02 |
| Heavy metals(pb) | ≤5ppm |
| Fe | ≤5ppm |
| Cl | ≤3ppm |
| Residue on ignition | ≤0.05% |
| Melting point | 168.0-172.0 |
(Molecular structure)
Configuration method of Tris solution:
1. Weigh the required amount of Tris powder and add it to sterile high purity water.
2. Use a magnetic stirrer to fully dissolve the Tris powder until it is completely homogeneous.
3. Calculation of concentration: Generally, the concentration of trimethylolaminomethane is 1 mol/L, and the concentration can be adjusted appropriately according to the need.
4. Measure the PH value of the solution with a PH meter, which should generally be controlled between 7.0-8.5.
5. Preparation of buffer solution: standardize the solution to the required volume, usually used as a buffer solution with a concentration of 50-200 mmol/L.
6. Sterilization: The trimethylolaminomethane solution is loaded into sterile bottles and sterilized according to a certain process.
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