1. Oxidation reaction: In Tris buffer, the hydroxyl group in the Tris molecule may undergo oxidation reaction, generating colored products, resulting in the color change of the solution, the specific mechanism of this reaction includes the oxidation of the hydroxyl group in the Tris molecule to form an aldehyde group, and then the aldehyde group and other Tris molecules condense to produce colored products, often blue or purple, resulting in the entire Tris buffer turning blue.
2. Effect of oxidants: In the laboratory, common oxidants such as hydrogen peroxide (H₂O₂) may exist in Tris buffer, which can accelerate the oxidation reaction of Tris, resulting in significant color changes. Care needs to be taken to avoid inappropriate contact with oxidants when preparing Tris buffers.
3. Light and air oxidation: The color change of Tris buffer may also be related to light and air oxidation. Prolonged exposure to light and air accelerates the oxidation reaction of Tris and changes in color, so it is important to avoid prolonged exposure to light and air when preparing and storing Tris buffers.
4. Influence of metal ions: Some metal ions may participate in the oxidation reaction of Tris as catalysts, accelerate the generation of colored products, and if there is metal ion contamination in the Tris buffer, it may also lead to color changes.
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