Organic Intermediate Dithiothreitol CAS 3483-12-3 For Protein

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Dithiothreitol, also known by synonyms such as Dithiothreitol, threo - 1,4 - Dimercapto - 2,3 - butanediol, Cleland's reagent, and abbreviated as DTT, has the CAS No. 3483 - 12 - 3. Its molecular formula is C4H10O2S2 with a molecular weight of 154.25. It presents as a white free - flowing and hygroscopic powder and has an assay of 98%.

Application

• Dithiothreitol is as a reducing agent and deprotecting agent for thiolated DNA. The thiolated DNA terminal sulfur atom tends to form a dimer in solution, especially in the presence of oxygen.
• Dithiothreitolis also often used for the reduction of disulfide bonds in proteins and can be used to prevent intramolecular or intermolecular disulfide bonds of proteins formed between cysteines in proteins. However, DTT often cannot reduce the disulfide bonds embedded in the protein structure (solvent unreachable). The reduction of such disulfide bonds often requires denaturation of the protein (heating at high temperature or adding a denaturing agent such as 6M guanidine hydrochloride, 8M urea or 1% SDS).

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